SAFETY ASSESSMENT OF SOYBEANS EXPOSED TO GLYPHOSATE USING IN VIVO ASSAY

Abstract

Soybean (Glycine max (L.) merrill) is the main economic crop in Paraguay, where conventional and/or transgenic varieties are planted. Possible side effects of GM soy are still under discussion The aim of this study was to evaluate the mutagenic effect of three varieties of soybean two transgenic BRS 245 RR; Nueva Andrea 66R (T1, T2) and a conventional BRS 232 (T3) exposed to Glyphosate in the diet of mice using in vivo trials. Methods: tests were carried out in a murine model. Two trials were performed, first feeding BALB/C mice for 14 days and 42 days with T1 and T3, then feeding Swiss albino mice for 14 days with T2 and T3. Treatments were done in two groups: without glyphosate (G0) and with glyphosate (G1). The animals treated for 14 days were tested for Micronucleus and the animals treated for 42 days were tested for Micronucleus and Chromosomal Aberration. Results: Both trials had no mutagenic effects in mice. It is concluded that the soybean varieties evaluated did not present mutagenic effects (aneugenic or clastogenic) under these experimental conditions.